P2Y11 Purinoceptor Mediates the ATP-Enhanced Chemotactic Response of Rat Neutrophils
نویسندگان
چکیده
منابع مشابه
P2Y11 purinoceptor mediates the ATP-enhanced chemotactic response of rat neutrophils.
ATP and hydrolysis products of ATP like adenosine regulate the chemotaxis of neutrophils by activating purinoceptors and adenosine receptors. The present study was designed to examine exogenous ATP, activation of purinoceptors, and activation of A(3) adenosine receptor as key steps in the signal cascades that control cell orientation and migration of rat neutrophils. One or more of those steps ...
متن کاملHeterologous expression of a P2x-purinoceptor in rat chromaffin cells detects vesicular ATP release.
A cloned P2x-purinoceptor was transiently expressed in single isolated rat adrenal chromaffin cells and evaluated for the detection of released ATP. After cytoplasmic injection of the P2x complementary RNA (cRNA; 4-24 h), application of ATP produced an inwardly rectifying current over the voltage range -130 to -10 mV as measured by the whole cell patch-clamp technique. The dose-response curve f...
متن کاملTime-dependent alterations in the deformability of human neutrophils in response to chemotactic activation.
Transit times of individual human neutrophils through single capillary-sized pores were measured to determine the time-dependent changes in the mechanical behavior of the cells during activation by the chemotactic agent formyl-methionyl-leucyl-phenylalanine (FMLP) and in response to cytochalasin B (CTB) and colchicine. FMLP elicited a two-phase response consisting of a rapid increase in cell st...
متن کاملThe Response of Human Neutrophils to a Chemotactic Tripeptide ( N - Formyl - Methionyl - - Leucyl - Phenylalanine )
A quantitative microcinematographic technique for the measurement of cellular motion in response to chemical influences is described. The data are analyzed by using a magnetic digitizer connected to a computer. permitting comparison of several characteristics of such motion. When human granulocytes are placed in a gradient of formyl-methionyl-Ieucyl-phenylalanine (fMLP) (concentration 1O6_1O8 m...
متن کاملSulfasalazine inhibits the synthesis of chemotactic lipids by neutrophils.
Neutrophils metabolize arachidonic acid through the liposygenase pathway to 5-hydroxy-6,8,11,14-eicosatetrenoic acid (5-HETE) and 5,12-dihydroxy-6,8,10,14-eicosatraenoic acid (5,12 diHETE). 5-HETE and 5,12diHETE are potent chemotactic agents and are thought to have important roles in the inflammatory response. In this study we demonstrate the sulfasalazine, at concentrations found in the stool ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Pharmacological Sciences
سال: 2012
ISSN: 1347-8613,1347-8648
DOI: 10.1254/jphs.12173fp